10.1371/journal.pcbi.1003371.g003
Eduard J. Kerkhoven
Eduard
J. Kerkhoven
Fiona Achcar
Fiona
Achcar
Vincent P. Alibu
Vincent P.
Alibu
Richard J. Burchmore
Richard
J. Burchmore
Ian H. Gilbert
Ian H.
Gilbert
Maciej Trybiło
Maciej
Trybiło
Nicole N. Driessen
Nicole
N. Driessen
David Gilbert
David
Gilbert
Rainer Breitling
Rainer
Breitling
Barbara M. Bakker
Barbara
M. Bakker
Michael P. Barrett
Michael P.
Barrett
ATP:ADP antiporter mimics turbo-state.
Public Library of Science
2013
antiporter
mimics
2013-12-05 02:47:01
Figure
https://plos.figshare.com/articles/figure/ATP_ADP_antiporter_mimics_turbo_state_/869002
<p>(<i>A</i>) Overview of the models used in this figure. Model A and D are from <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g001" target="_blank">Figure 1</a>, model A–glyc is model A without glycosomal localization, as described in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371-Haanstra2" target="_blank">[31]</a>, model A+AAT is model A with an ATP:ADP antiporter. (<i>B–C</i>) Steady-state concentrations of glycosomal Glc-6-P and Fru-1,6-BP are depicted in the various models. (<i>D</i>) Increasing the activity of the ATP:ADP antiporter (V<sub>max,ATP:ADP antiporter</sub>) in model D leads to a high risk of accumulation of hexose phosphates. The green line indicates the concentration of Fru-1,6-BP in the original model of glycolysis (17.2 mM, panel C, model A). Glc<sub>e</sub> in this simulation is 25 mM. (<i>E</i>) Time course simulation of model D at 25 mM Glc<sub>e</sub> and various values for the V<sub>max,ATP:ADP antiporter</sub> parameter. Plotted is the concentration of glycosomal phosphates (ΣP similar as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>, moiety 5 in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-t002" target="_blank">Table 2</a>). ATP:ADP antiporter activity values below 1 nmol·min<sup>−1</sup>·mg protein<sup>−1</sup> result in depletion of glycosomal phosphates (cf. <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>). <i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup>·mg protein<sup>−1</sup> in all models. Solid lines indicate medians, shaded areas and error bars show interquartile ranges, as derived from the uncertainty modeling.</p>