10.1371/journal.pcbi.1003371.g003 Eduard J. Kerkhoven Eduard J. Kerkhoven Fiona Achcar Fiona Achcar Vincent P. Alibu Vincent P. Alibu Richard J. Burchmore Richard J. Burchmore Ian H. Gilbert Ian H. Gilbert Maciej Trybiło Maciej Trybiło Nicole N. Driessen Nicole N. Driessen David Gilbert David Gilbert Rainer Breitling Rainer Breitling Barbara M. Bakker Barbara M. Bakker Michael P. Barrett Michael P. Barrett ATP:ADP antiporter mimics turbo-state. Public Library of Science 2013 antiporter mimics 2013-12-05 02:47:01 Figure https://plos.figshare.com/articles/figure/ATP_ADP_antiporter_mimics_turbo_state_/869002 <p>(<i>A</i>) Overview of the models used in this figure. Model A and D are from <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g001" target="_blank">Figure 1</a>, model A–glyc is model A without glycosomal localization, as described in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371-Haanstra2" target="_blank">[31]</a>, model A+AAT is model A with an ATP:ADP antiporter. (<i>B–C</i>) Steady-state concentrations of glycosomal Glc-6-P and Fru-1,6-BP are depicted in the various models. (<i>D</i>) Increasing the activity of the ATP:ADP antiporter (V<sub>max,ATP:ADP antiporter</sub>) in model D leads to a high risk of accumulation of hexose phosphates. The green line indicates the concentration of Fru-1,6-BP in the original model of glycolysis (17.2 mM, panel C, model A). Glc<sub>e</sub> in this simulation is 25 mM. (<i>E</i>) Time course simulation of model D at 25 mM Glc<sub>e</sub> and various values for the V<sub>max,ATP:ADP antiporter</sub> parameter. Plotted is the concentration of glycosomal phosphates (ΣP similar as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>, moiety 5 in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-t002" target="_blank">Table 2</a>). ATP:ADP antiporter activity values below 1 nmol·min<sup>−1</sup>·mg protein<sup>−1</sup> result in depletion of glycosomal phosphates (cf. <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi-1003371-g002" target="_blank">Figure 2</a>). <i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup>·mg protein<sup>−1</sup> in all models. Solid lines indicate medians, shaded areas and error bars show interquartile ranges, as derived from the uncertainty modeling.</p>