10.1371/journal.pcbi.1003371.g005
Eduard J. Kerkhoven
Eduard
J. Kerkhoven
Fiona Achcar
Fiona
Achcar
Vincent P. Alibu
Vincent P.
Alibu
Richard J. Burchmore
Richard
J. Burchmore
Ian H. Gilbert
Ian H.
Gilbert
Maciej Trybiło
Maciej
Trybiło
Nicole N. Driessen
Nicole
N. Driessen
David Gilbert
David
Gilbert
Rainer Breitling
Rainer
Breitling
Barbara M. Bakker
Barbara
M. Bakker
Michael P. Barrett
Michael P.
Barrett
Simulations of 6PGDH inhibition and 6-PG accumulation.
Public Library of Science
2013
6pgdh
inhibition
6-pg
2013-12-05 02:47:01
Figure
https://plos.figshare.com/articles/figure/Simulations_of_6PGDH_inhibition_and_6_PG_accumulation_/869004
<p>(<i>A–B</i>) The effects of inhibition of 6PGDH on 6-PG concentrations and metabolic fluxes were simulated by reducing <i>V</i><sub>max,6PGDH</sub> in model C and D at high oxidative stress (<i>k<sub>TOX</sub></i> = 200 µl·min<sup>−1</sup>·mg protein<sup>−1</sup>). Simulations at low oxidative stress (<i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup>·mg protein<sup>−1</sup>) are shown in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371.s009" target="_blank">Figure S6</a>. ATP production flux as steady-state flux through PFK is indicated in red, while trypanothione reductase steady-state flux is indicated in yellow, both plotted on the left y-axis. Steady-state concentration of cytosolic (blue) and glycosomal (green) 6-phosphogluconate are plotted on the right y-axis. Shaded areas indicate interquartile ranges. (<i>C</i>) Steady-state flux through glycolysis as a function of the glycosomal 6-PG concentration in model A. A glycosomal 6-PG concentration of around 500 mM reduces the glycolytic flux by 50%.</p>