10.1371/journal.pcbi.1003371.g005 Eduard J. Kerkhoven Eduard J. Kerkhoven Fiona Achcar Fiona Achcar Vincent P. Alibu Vincent P. Alibu Richard J. Burchmore Richard J. Burchmore Ian H. Gilbert Ian H. Gilbert Maciej Trybiło Maciej Trybiło Nicole N. Driessen Nicole N. Driessen David Gilbert David Gilbert Rainer Breitling Rainer Breitling Barbara M. Bakker Barbara M. Bakker Michael P. Barrett Michael P. Barrett Simulations of 6PGDH inhibition and 6-PG accumulation. Public Library of Science 2013 6pgdh inhibition 6-pg 2013-12-05 02:47:01 Figure https://plos.figshare.com/articles/figure/Simulations_of_6PGDH_inhibition_and_6_PG_accumulation_/869004 <p>(<i>A–B</i>) The effects of inhibition of 6PGDH on 6-PG concentrations and metabolic fluxes were simulated by reducing <i>V</i><sub>max,6PGDH</sub> in model C and D at high oxidative stress (<i>k<sub>TOX</sub></i> = 200 µl·min<sup>−1</sup>·mg protein<sup>−1</sup>). Simulations at low oxidative stress (<i>k<sub>TOX</sub></i> = 2 µl·min<sup>−1</sup>·mg protein<sup>−1</sup>) are shown in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003371#pcbi.1003371.s009" target="_blank">Figure S6</a>. ATP production flux as steady-state flux through PFK is indicated in red, while trypanothione reductase steady-state flux is indicated in yellow, both plotted on the left y-axis. Steady-state concentration of cytosolic (blue) and glycosomal (green) 6-phosphogluconate are plotted on the right y-axis. Shaded areas indicate interquartile ranges. (<i>C</i>) Steady-state flux through glycolysis as a function of the glycosomal 6-PG concentration in model A. A glycosomal 6-PG concentration of around 500 mM reduces the glycolytic flux by 50%.</p>